determination of lethal dose of plant extract
determination of lethal dose of plant extract
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determination of lethal dose of plant extract

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determination of lethal dose of plant extract

determination of lethal dose of plant extract-[mining plant] Safety evaluations of the aqueous extract of the leaves of Moringa ... lethal levels of cyanide. HCN has a high affinity ... The plant extract did not produce any ... Analysis of the procedure for determining the LD50.

Acute toxicity studies and determination of median lethal dose

The aqueous root extract of Lecaniodiscus cupanioides was used to study the toxicity pattern of the plant. The extract was found to be non-toxic up to 20g/kg, when given by the oral route to mice ...

Determining LD50 for a plant extract - ResearchGate

The dosages of the plant extracts were doubled for instance 2mg, 4mg,8 mg 16 mg etc. The dosage that killed 50% of the experimental animals within 24 h was taken as the LD50 value Cite

DETERMINATION OF MEDIAN LETHAL DOSE OF ETHANOLIC

Packaging of Extract/Weight A spatula was used to collect the extract into a 20ml weighed beaker (pyrex), covered with a foil paper and preserved at normal room temperature. The weight of the extract was determined using a chemical balance. Determination of the Median Lethal Dose The median lethal dose of the ethanolic extract of

(PDF) ACUTE TOXICITY (LETHAL DOSE 50 CALCULATION) AND ...

The test of acute toxicity (LD 50 ) is used to determine the range of doses and evaluating the therapeutic index of plant extract (Ahmed and Azmat, 2014). In the current study, the acute toxicity ...

Acute toxicity studies and determination of median lethal dose

This study investigated the toxic effects of crude fruit endocarp extract of L. siceraria on Clarias gariepinus juveniles by exploring the proximate analyses of the plant, and the behavioural ...

determination of half lethal dose - Chitawon Park Cottage

determination of lethal dose of plant extract[mining plant] ... The LD 50 is the dose that kills half (50%) of the animals tested (LD = "lethal dose"). The animals are usually rats or mice, although rabbits, ... Plant extract: 60: Prepare and Analyze a Plant Extract by GC LEAP. Get Price

Acute toxicity studies and determination of median lethal dose

This study investigated the toxic effects of crude fruit endocarp extract of L. siceraria on Clarias gariepinus juveniles by exploring the proximate analyses of the plant, and the behavioural ...

Acute Toxicity (Lethal Dose 50 Calculation) of Herbal Drug ...

arithmetic method of Karber [15] was used for the determination of LD50. LD50 LD100 = −∑ ×(a bn) n = total number of animal in a group. a = the difference between two successive doses of administered extract/substance. b = the average number of dead animals in two successive doses. LD100 = Lethal dose causing the 100% death of all test ...

Lethal Concentration - an overview ScienceDirect Topics

16.8.4.1 Lethal Concentration Determination The lethal concentrations of test drugs/plant extract resulting in 50% mortality of the brine shrimp (LC 50 ) and 95% confidence intervals are determined from the 24 and 48-h counts and the dose–response data are transformed into a straight line by means of a trend line fit linear regression ...

EVALUATION OF THE LETHAL DOSE OF THE METHANOL

The largest dose that did not kill any rat was noted, as well as the smallest dose that killed all the animals. Acute Toxicity Screening This study was done to determine the median lethal dose (LD 50) of the methanol extract of Rhizophora racemosa. A total

Evaluation of acute and subacute toxicity of whole-plant ...

Results: In the acute toxicity study, a single dose of the aqueous extract at 2000 or 5000 mg/kg caused no mortality in the animals, suggesting that the median lethal dose is greater than 5000 mg/kg. In the subacute toxicity study, administration of the extract for 28 d, at all doses, caused no significant changes in the body weights or organ ...

Sapium ellipticum (Hochst.) Pax Leaf Extract In-vitro ...

Prior to this investigation, the LD50 and antioxidant activities of Sapium ellipticum leaf extract have not been reported, hence; the focus of this study. The LD50 of the extract

Tanacetum parthenium L. in albino wistar rats

the plant extract, acute oral toxicity is the first step to [13]be carried out . Acute toxicity testing involves the estimation of lethal dose, the dose that kills 50% of the tested group of animals.

Antidiarrhoea and toxicological evaluation of the leaf ...

Nov 17, 2010  The crude ethanol extract and fractions inhibited the growth of E. coli, P. aeruginosa, S. aureus and S. typhi to varying extents. The degree of transition exhibited by the charcoal meal was dose-dependent. In the castor oil induced diarrhoea test, all the doses showed anti-spasmodic effects. The LD50 in mice was above 500 mg/kg body weight.

Frontiers Antimicrobial Properties and Mechanism of ...

Jul 24, 2018  Where X 1 refers to the weight of extract after evaporation of solvent and X 0 refers to the dry weight of the plant powder before extraction.. Preparation of Inoculum. The antimicrobial properties of plant extracts were tested against Gram-positive bacteria [Bacillus cereus 10451 (BC), Staphylococcus aureus 10786 (SA)], Gram-negative bacteria [Escherichia coli GIM1.708 (EC),

Acute Toxicity Study and Determination of Median Lethal ...

This research was carried out to evaluate the acute toxic effects of ethanol leaves extract of C. roseus and to determine its median lethal dose (LD 50). The objective was to investigate the toxicity of the leaves extract on the liver and biochemical parameters in rats.

Acute Toxicity and Lethality of Gladiolous psittacinus

Determination of the median lethal dose (LD50) of the plant extract was done in two phases. In the first phase, nine rats were divided into three groups of three rats each and were treated with the methanol extract of the plant at dosages of 10, 100 and 1000mg/kg body weight orally. They were observed for 24 hours for signs of toxicity.

Immunomodulatory and Anticancer Activities of Hyacinthus ...

Mar 24, 2021  The maximum non-lethal and minimum lethal doses represented the lower and upper limits, which were used to prepare LD₅₀ doses . For LD₅₀ determination, four groups ( n = 4) of mice were injected intraperitoneally with different concentrations (2500, 3000, 4000, and 5000 mg/kg) of the extract within the higher and lower range detected in ...

In Vitro Anthelmintic Activity of Ethanol Stem Bark ...

Albizia ferruginea (Guill. amp; Perr.) Benth bark is used in the traditional medicine as a vermifuge. This study sought to determine the anthelmintic activity of the stem bark extract of Albizia ferruginea. The powdered A. ferruginea stem bark was extracted with ethanol. Phytochemical screening was carried out on A. ferruginea ethanol extract (AFE) and then ...

determination of half lethal dose - Chitawon Park Cottage

determination of lethal dose of plant extract[mining plant] ... The LD 50 is the dose that kills half (50%) of the animals tested (LD = "lethal dose"). The animals are usually rats or mice, although rabbits, ... Plant extract: 60: Prepare and Analyze a Plant Extract by GC LEAP. Get Price

Acute Toxicity Study and Determination of Median Lethal ...

This research was carried out to evaluate the acute toxic effects of ethanol leaves extract of C. roseus and to determine its median lethal dose (LD 50). The objective was to investigate the toxicity of the leaves extract on the liver and biochemical parameters in rats.

Antibacterial activity of plant extracts from northwestern ...

Methods and Results: Plant extracts were prepared according to traditional uses in northwestern Argentina. Antibacterial activity was assayed by agar dilu-tion in Petri dishes and broth dilution in 96-well plates. Lethal dose 50 (LD 50) was determined by the Artemia salina assay. Phytochemical analysis was

Antidiarrhoea and toxicological evaluation of the leaf ...

Nov 17, 2010  The crude ethanol extract and fractions inhibited the growth of E. coli, P. aeruginosa, S. aureus and S. typhi to varying extents. The degree of transition exhibited by the charcoal meal was dose-dependent. In the castor oil induced diarrhoea test, all the doses showed anti-spasmodic effects. The LD50 in mice was above 500 mg/kg body weight.

Acute Toxicity and Lethality of Gladiolous psittacinus

Determination of the median lethal dose (LD50) of the plant extract was done in two phases. In the first phase, nine rats were divided into three groups of three rats each and were treated with the methanol extract of the plant at dosages of 10, 100 and 1000mg/kg body weight orally. They were observed for 24 hours for signs of toxicity.

Chapter IV. Guidelines for Toxicity Tests

animals, rather than on determining the median lethal dose (LD 50) of the substance. ... Determination of the approximate lethal dose with about six anima ls. J.Indust. Hygiene Toxicol. 25:415-417 ...

Toxicity of Turmeric Extracts to the Termite ...

However, in continuous exposure assays, the LC(50) for hexane extracts of crude turmeric powder was 9.6 mg, or 1.0 mg starting material per square centimeter of filter paper substrate. These active components were soluble in a range of polar and apolar solvents, but only hexane could selectively fractionate active components away from the ...

Determination of lethal dose (LD50) of Exotoxin A from ...

on elongation factor 2 and was lethal at a dose of 1μg when injected in to laboratory mice. While [15,8 ] noted that the purified toxin had a mouse median lethal dose of 0.1- 0.15 μg /22g mouse when injected intraperitoneally, also Michael and Barbara [16] reported that LD50 of toxin A was 0.2 μg/ ml when

Paradoxical Effects of Methanol Extracts of ...

Oct 11, 2020  The median lethal doses of the two forms of extracts were determined by the method described by Lorke. The qualitative phytochemical screening of the extracts showed relative presence of alkaloids, flavonoids, hydrogen cyanides, steroids, soluble carbohydrates, tannins and phenol in the fermented and unfermented extracts;

Oral and intraperitoneal LD50 of thymoquinone, an active ...

Background: Thymoquinone is the major active principle of Nigella sativa (N. sativa) and constitutes about 30% of its volatile oil or ether extract. N. sativa oil and seed are commonly used as a natural remedy for many ailments. Using modern scientific techniques, a number of pharmacological actions of N. sativa have been investigated including immunostimulant, anti-inflammatory, anticancer ...

THERAPEUTIC EFFECT OF TAMARINDUS INDICA EXTRACTS

Determination of Lethal Dose (LD 50): LD 50 of the plant extract was determined by the method 7 using thirty-three rats (Rattus norvegicus) weighing between 200-220g. The first phase, 15 rats were divided into five groups (each group contains three rats) treated with aqueous extract at doses of 125, 250, 500, 750 and 1000 mg/kg body weight.

In – Vitro activity of Desmostachya bipinnata (L.) Stapf ...

May 01, 2018  The dried plant extract was freshly suspended in distilled water just before administration. 2.4.2. Determination of median lethal dose (LD 50) LD 50 of the ethanol extract was determined as described before (El-Meligy et al., 2017). For this purpose, 5 groups of 5 mature mail Swiss albino mice (23–25 g body weight) each were used.

Adaptogenic and safety evaluation of seabuckthorn ...

The effects of seabuckthorn (Hippophae rhamnoides L., Elaeagnaceae), leaf aqueous extract were examined in rats for its adaptogenic activity and toxicity. Dose dependent adaptogenic study of extract was carried out at different doses administered orally, 30min prior to cold (5 degrees C)-hypoxia (42

Effect of arsenic trioxide on renal functions and its ...

The protective effects of Curcuma aromatica leaf extract were studied on nehrotoxicity induced by arsenic trioxide in albino rats. LD50 estimated for arsenic trioxide was 14.98 mg kg(-1) body weight. Nephrotoxicity was assessed by estimating the serum levels of urea, uric acid and creatinine, the ma